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Phosphatase substrate ambiguity. SerB is the phosphoserine phosphatase that catalyzes the final step in the biosynthesis of serine from 3-phosphoglycerate (top). Deletion of serB can be rescued by expression of 2 other HAD-like phosphatases: Gph, which hydrolyzes the phosphate monoester 2-phosphoglycolate (middle), and HisB, which ordinarily catalyzes the phosphorolysis of histidinol phosphate (bottom).
