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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Drug Metab Dispos. 2008 Sep 17;36(12):2506–2512. doi: 10.1124/dmd.108.022723

Figure 3. Regulation of hepatic CYP3A4 in TgCYP3A4/hPXR mice.

Figure 3

TgCYP3A4 and TgCYP3A4/hPXR mice (8-week-old) were treated for three days with corn oil, 10 mg/kg/day PCN (ip), or 10 mg/kg/day RIF (po) as detailed under Materials and Methods. Liver was collected from transgenic male (A) and female (B) mice 24 h after the last dose. Pooled liver microsomes (3 samples) were prepared and analyzed by western blot. The monoclonal antibody against CYP3A4 (mAb 275-1-2) recognizes human CYP3A4 but not mouse Cyp3a or other liver proteins. Human liver microsome (HLM) served as positive control for CYP3A4. GAPDH was used as loading control.