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. Author manuscript; available in PMC: 2009 May 7.
Published in final edited form as: Biochemistry. 2007 Dec 8;47(1):320–330. doi: 10.1021/bi701472g

Fig. 6.

Fig. 6

Sialylation of Globo backbone structures by reverse sialylation of ST3Gal-II. Incubation mixtures (500μl) contained [9-3H]Sialyl Fetuin (5 mg), 200mM NaCacodylate pH 6.0, 20mM 5′-CMP and the following: a. 50 mU ST3Gal-II and b. 6.0 mM Galβ1,3GalNAcβ1,3Galα-O-Me and 50mU ST3Gal-II. Reaction products were isolated using Biogel P2 column after 20h at 37ºC and product identity was verified by mass spectrometry as indicated by the molecular weights in the panel. Peak I: Unused [9-3H]Sialyl Fetuin; Peak II: [9-3H]Sialyl product from acceptor; Peak III: CMP-[9-3H]NeuAc from 5′-CMP.