. Author manuscript; available in PMC: 2009 May 7.

Published in final edited form as: Biochemistry. 2007 Dec 8;47(1):320–330. doi: 10.1021/bi701472g

Table 2.

^* Incorporation of [9-³H]NeuAc from donors ^**

Acceptor (2.5mM)		[9-H]NeuAcα2,3Galβ1,3(GlcNAcβ1,6)GalNAcα-O-Al [[9-³H][1]]	[9-³H]NeuAcα2,3Galβ1,3(6-O-sulfoGlcNAcβ1,6) GalNAc-O-Al [[9-³H][4]	[9-³H]NeuAcα2,3Galβ1,3GalNAcβ1,3Galα-O-Me[[9-³H][3]]

D-Fucβ1,3GalNAcα-O-Bn	[5]	100.0 (34569 CPM)	100.0 (97675 CPM)	100.0 (6224 CPM)^***
D-Fucβ1,3(GlcNAcβ1,6)GalNAcα-O-Bn	[6]	ND	96.9	106.5
4-FGalβ1,3GalNAcα-O-Bn	[7]	60.8	33.3	4.1
Galβ1,3(6-O-Me)GalNAcα-O-Bn	[8]	43.0	21.5	3.3
3-O-MeGalβ1,4GlcNAcβ1,6(Galβ1,3)	[9]			ND
GalNAcα-O-Bn		22.4	10.0
Galβ1,4GlcNAcβ1,6(Galβ1,3)	[10]
GalNAc3-O-Bn		20.5	8.4	ND
4-O-MeGalβ1,3GalNAcα-O-Bn	[11]	8.9	2.7	0.2
Galβ1,4GlcNAcβ-O-Bn	[12]	ND	1.9	0.5
Galβ1,4GlcNAcβ1,6(4-O-MeGalβ1,3)	[13]
GalNAcα-O-Bn		1.3	0	ND

ND: Not Determined

150μM [9-³H] labeled donor was incubated with 1mM 5′-CMP and 2.5mM either T-hapten or mucin core-2 based acceptor in the presence of 0.2mU ST3Gal-II for 4h. under reaction conditions identical to Fig. 1. Acceptors were separated from donor using C-18 cartridge due to hydrophobicity of Bn (Benzyl) group. Radioactivity of eluate was quantified. The blank containing no acceptor had CPM<100.

^**

Incorporation of [9-³H]NeuAc expressed as percent of the CPM incorporated into [5]

^***

CPM for this donor is low since 10 fold cold CMP-NeuAc (i.e., lower specific radioactivity of CMP-[9-³H]NeuAc) was used to synthesize it as compared to other two donors.