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. Author manuscript; available in PMC: 2009 May 7.
Published in final edited form as: Biochemistry. 2007 Dec 8;47(1):320–330. doi: 10.1021/bi701472g

Table 4.

* Reversible sialyltransferase activity in human cancer cell lines

Cancer Cell Lines Transfer of [9-3H]NeuAc from [9-3H[4]] to [5]
CPM ×10−3/mg protein
BREAST:
 T47D 0.52
 ZR75-1 1.32
 MDA-MB231 0.09
 MDA-MB-435S 0.69
 MCF-7 0.57
COLON:
 LS180 0.01
PROSTATE:
 LNCaP 19.89
 PC3 4.72
LEUKEMIA
 HL60 0.76
*

0.15mM [9-3H]NeuAcα2,3Galβ1,3(6-O-sulfo GlcNAcβ1,6) GalNAc-O-Al [9-3H[4]] was added to 3.0mM D-Fucβ1,3GalNAcα-O-Bn [5] in the presence of 1.0mM 5′-CMP and 100 μl Triton-X solubilized cell extract for 16h. at 37ºC at pH 6.0. Total reaction volume was 180 μl. The product [9-3H]NeuAcα2,3D-Fucβ1,3GalNAcα-O-Bn was measured using Sep-Pak C18 separation followed by liquid scintillation counting.