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Oncrasin-induced aggregation of PKCι T29, T29Kt1, and H460 cells were treated with DMSO or 10 μM (for T29 or T29Kt1) or 1 μM (for H460) oncrasin-1 for 12 hours, and then immunofluorescent staining was performed to test for intracellular localization of PKCι. Nuclear PKCι aggregated into large foci after oncrasin-1 treatment in sensitive T29Kt1 and H460 cells but not in the resistant T29 cells.
