Figure 6.

Activation of εPKC increases phosphorylation of hippocampal mitochondrial electron transport chain proteins in rats subjected to ischemic preconditioning. Hippocampal synaptosomes were isolated at 48 h of reperfusion from sham (n = 6) or IPC-treated (n = 6) animals. The extent of 18 kDa subunit of complex I (A), Fe-S subunit of complex III (B), and COX IV subunit of complex IV (C) phosphorylation was measured in synaptosomes treated with tat or ψεRACK in both experimental groups by immunoprecipitation using anti-phospho-serine, anti-phospho-threonine, and anti-phospho-tyrosine antibodies followed by Western blotting using anti-18 kDa complex I, anti Fe-S subunit of complex III, and anti-COX IV subunit of complex IV antibody, respectively. The results are expressed as mean ± SEM of percentage of phosphorylation in ψεRACK-treated synaptosomes isolated from hippocampus of sham-treated animals. *p < 0.05, IPC versus sham.