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Formation of amylo-1,6-glucosidase,4-α-glucanotransferase (AGL) mutants. (A) AGL mutants form aggresomes. Cells were transfected with HA-tagged AGL constructs for 12 h, and then 10 µm MG-132 was included during the media change and cells were allowed to continue for an additional 12 h before immunofluorescence analysis. AGL proteins were immunostained with HA-antibodies and the nuclei were visualized with TOPRO3.
