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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Mol Pharm. 2009;6(1):158–172. doi: 10.1021/mp800111a

Figure 1.

Figure 1

Various constructs of HB290 scFv [(A) VH-VL and (B) VL-VH] and bfFp [(C) pWET5, (D) pWET6 and (E) pWET7]. The genes were cloned in different orientations to select the best production in E. coli. Abbreviations: pelB, bacterial leader sequence; VL, variable domain light chain; VH, variable domain heavy chain; CH1-p, partial constant heavy chain region 1, 15 amino acid linker; CL-p, partial constant light chain region, 15 amino acid linker; G, glycine; S, serine; His6, hexa histidine amino acid tag.