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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Mol Pharm. 2009;6(1):158–172. doi: 10.1021/mp800111a

Figure 4.

Figure 4

Analysis of humoral immune responses to biotinylated antigens in vivo. Groups of five mice were immunized with different antigen combinations along with a PBS control. The specific components, amounts, and the schedule of immunizations are outlined in Table 3. A simplified form of the immunization components is listed in the figure legend. (A) Versatility of bfFp based delivery of protein, peptide, glycolipids and DNA to DC. (B) bfFp mediated delivery of other infectious diseases viral DNA to DC. (C) Multiple antigen delivery to DC using bfFp. The mice were analyzed individually, and the data was pooled. The humoral response, as quantified by serum antibody titers on day 21 post immunization, was measured by ELISA method against the respective antigens shown in X-axis (listed in Table 2). The method involved coating of 100 μL of 10 μg/mL antigen in microtiter plates followed by addition of 1:1000 diluted serum antibody, and detection using GAM-HRPO. The ELISA measurements were done in quadruplicate for each mouse. The mean ELISA values obtained for each individual mouse were further averaged. The error bars represent the standard deviation in a group of 5 mice.