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. Author manuscript; available in PMC: 2010 May 15.

Published in final edited form as: Chem Biol Interact. 2008 Dec 30;179(2-3):202–211. doi: 10.1016/j.cbi.2008.12.014

Fig. 3 — Inhibition of nitric oxide production in LPS-activated RAW 264.7 cells by isothiocyanates (A). Cells were treated with LPS (1 μg/ml) for 24 h in the presence of different concentrations of PEITC or ITC-1 or ITC-8 or ITC-9. The culture supernatants were subsequently analyzed for nitrite production. The data were percentage of control values (LPS-stimulated cells in the absence of ITCs). Each point represents the mean ± S.D. of three independent assays. *Significant difference from control (0.1% ethanol) at P < 0.05. Suppression of iNOS protein (B) and mRNA (C) expression by ITCs. The protein expression of iNOS and GAPDH was monitored after 12 h treatment with vehicle control (0.1% ethanol) or LPS (1 μg/ml) or combination of LPS/PEITC or LPS/ITC-1 or LPS/ITC-8 or LPS/ITC-9 using immunoblotting. The mRNA expression of iNOS and GAPDH was quantified by RT-PCR analysis after 6 h incubation. Cells were treated as described in (B).

Fig. 3 — Inhibition of nitric oxide production in LPS-activated RAW 264.7 cells by isothiocyanates (A). Cells were treated with LPS (1 μg/ml) for 24 h in the presence of different concentrations of PEITC or ITC-1 or ITC-8 or ITC-9. The culture supernatants were subsequently analyzed for nitrite production. The data were percentage of control values (LPS-stimulated cells in the absence of ITCs). Each point represents the mean ± S.D. of three independent assays. *Significant difference from control (0.1% ethanol) at P < 0.05. Suppression of iNOS protein (B) and mRNA (C) expression by ITCs. The protein expression of iNOS and GAPDH was monitored after 12 h treatment with vehicle control (0.1% ethanol) or LPS (1 μg/ml) or combination of LPS/PEITC or LPS/ITC-1 or LPS/ITC-8 or LPS/ITC-9 using immunoblotting. The mRNA expression of iNOS and GAPDH was quantified by RT-PCR analysis after 6 h incubation. Cells were treated as described in (B).