Figure 1.

Construction of the rRSV/mIFN-γ chimeric genome. The mIFN-γ cDNA (shaded rectangle, with initiation and termination codons shown on either side in lowercase) was modified to be flanked by RSV gene-start and gene-end signals as an XmaI fragment. This transcription cassette was inserted into an antigenome cDNA that previously had been modified by the insertion of an 8-nt XmaI linker (bold, italicized) into the unique StuI site (the two halves of the StuI site, AGG and CCT, are underlined) present in the G-F intergenic region. XmaI sites are underlined.