Table 3.
T50, Tto, Ttd and Ttd – Tto values for known oligonucleotides
| Oligonucleotide | T50 | Tto | Ttd | Ttd – Tto |
|---|---|---|---|---|
| Unmethylated | 79.07 ± 0.06 | 78.48 ± 0.05 | 80.60 ± 0.06 | 2.12 ± 0.07 |
| Partially methylated | 80.00 ± 0.26 | 78.85 ± 0.53 | 82.39 ± 0.17 | 3.54 ± 0.47 |
| Fully methylated | 82.10 ± 0.00 | 81.13 ± 0.05 | 84.29 ± 0.03 | 3.16 ± 0.15 |
| U/P | 79.27 ± 0.21 | 78.30 ± 0.05 | 81.47 ± 0.18 | 3.17 ± 0.15 |
| U/M | 80.17 ± 0.15 | 78.45 ± 0.11 | 83.36 ± 0.19 | 4.90 ± 0.21 |
U/P – unmethylated oligonucleotide spiked with partially methylated oligonucleotide.
U/M – unmethylated oligonucleotide spiked with fully methylated oligonucleotide.
All values are the mean ± standard deviation of 3 replicate reactions.