Table 4.
T50, Tto, Ttd and Ttd – Tto values for TIMP3 in OE33 cells treated with vehicle or 5-aza-2'-deoxycytidine
| Sample | T50 | Tto | Ttd | Ttd – Tto |
|---|---|---|---|---|
| U ref | 75.52 ± 0.07 | 74.74 ± 0.16 | 77.36 ± 0.08 | 2.62 ± 0.24 |
| M ref | 78.07 ± 0.23 | 77.26 ± 0.37 | 79.83 ± 0.02 | 2.57 ± 0.35 |
| veh 48 h | 76.87 ± 0.18 | 75.63 ± 0.29 | 79.37 ± 0.04 | 3.74 ± 0.29 |
| veh 72 h | 76.80 ± 0.11 | 75.56 ± 0.17 | 79.33 ± 0.07 | 3.77 ± 0.19 |
| veh 120 h | 76.89 ± 0.18 | 75.70 ± 0.33 | 79.34 ± 0.08 | 3.65 ± 0.36 |
| aza 48 h | 75.40 ± 0.66 | 73.84 ± 1.15 | 78.90 ± 0.09 | 5.05 ± 1.12 |
| aza 72 h | 75.54 ± 0.42 | 74.34 ± 0.75 | 78.69 ± 0.06 | 4.35 ± 0.74 |
| aza 120 h | 75.49 ± 0.33 | 74.38 ± 0.57 | 78.45 ± 0.19 | 4.07 ± 0.64 |
U ref – unmethylated reference.
M ref – methylated reference.
veh – OE33 esophageal adenocarcinoma cell line treated with vehicle for 48, 72 or 120 h.
aza – OE33 esophageal adenocarcinoma cell line treated with 1 μM 5-aza-2'-deoxycytidine for 48, 72 or 120 h.
Data shown for veh or aza treated OE33 are the mean ± standard deviation of triplicate reactions for each of triplicate cultures. Data shown for U ref and M ref are the mean ± standard deviation of triplicate reactions.