Table 5.
T50, Tto, Ttd and Ttd – Tto values for MGMT in FFPE CRC tissues
| Sample | T50 | Tto | Ttd | Ttd – Tto |
|---|---|---|---|---|
| U ref | 74.17 ± 0.04 | 73.64 ± 0.04 | 75.54 ± 0.04 | 1.90 ± 0.03 |
| M ref | 78.70 ± 0.25 | 78.06 ± 0.37 | 80.43 ± 0.15 | 2.36 ± 0.25 |
| No | 74.18 ± 0.09 | 73.54 ± 0.06 | 77.24 ± 1.01 | 3.71 ± 0.95 |
| Clonal | 75.84 ± 0.21 | 73.62 ± 0.17 | 81.12 ± 0.37 | 7.50 ± 0.31 |
| Complete | 76.17 ± 0.07 | 73.70 ± 0.09 | 80.32 ± 0.45 | 6.62 ± 0.40 |
U ref – unmethylated reference.
M ref – methylated reference.
No, Clonal and Complete – whole sections of FFPE CRC tissue with either no, clonal or complete loss of MGMT protein expression in tumor cells, as determined by immunohistochemistry.
All values are the mean ± standard deviation of 6 replicate reactions.