Table 3.
Mixed-model nested ANOVA for the relative expression levels (dCts). (Sex (male versus female), mated (virgin versus mated) and immune component (humoral versus cell mediated) were the main effects and function within immune system, gene family within function and paralogue within gene family were the nested effects. Whether a particular effect was fixed (F) or random (R) appears in brackets.)
| source | d.f. | MS | F-ratio | F | p-value |
|---|---|---|---|---|---|
| A. mating- (F) | 1 | 67.3 | A/M | 96.14 | 0.0002 |
| B. sex- (F) | 1 | 17.2 | B/I | 1.30 | 0.3059 |
| C. immune component- (F) | 1 | 7.4 | C/E | 0.04 | 0.8494 |
| D. functional group (imm)- (F) | 2 | 288.3 | D/E | 1.70 | 0.2734 |
| E. gene family (fun)- (R) | 5 | 170.0 | E/F | 0.53 | 0.7486 |
| F. paralogue (fam)- (R) | 6 | 321.0 | F/T | 91.29 | <0.0001 |
| G. sex×imm | 1 | 2.2 | G/I | 0.17 | 0.6972 |
| H. sex×fun (imm) | 2 | 24.3 | H/I | 1.84 | 0.2518 |
| I. sex×fam (fun) | 5 | 13.2 | I/J | 0.52 | 0.7551 |
| J. sex×par (fam) | 6 | 25.2 | J/T | 7.20 | <0.0001 |
| K. mat×imm | 1 | 35.2 | K/M | 50.29 | 0.0009 |
| L. mat×fun (imm) | 2 | 4.4 | L/M | 6.29 | 0.0431 |
| M. mat×fam (fun) | 5 | 0.7 | M/N | 0.58 | 0.7167 |
| N. mat×par (fam) | 6 | 1.2 | N/T | 0.34 | 0.9187 |
| O. sex×mat | 1 | 8.3 | O/R | 4.37 | 0.0908 |
| P. sex×mat×imm | 1 | 0.6 | P/R | 0.32 | 0.5960 |
| Q. sex×mat×fun (imm) | 2 | 1.9 | Q/R | 1.00 | 0.4226 |
| R. sex×mat×fam (fun) | 5 | 1.9 | R/S | 0.90 | 0.5362 |
| S. sex×mat×par (fam) | 6 | 2.1 | S/T | 0.60 | 0.7303 |
| T. error | 281 | 3.5 |