Table 1.

Haemolysis assay. (Haemolysis (+) was determined by comparing the colour of the supernatant in each assay with the colour of the supernatant resulting from the incubation erythrocytes in 3 mM CaCl₂ double distilled water (ddH₂O; assay no. 1). A null level of haemolysis (−) was defined as the colour of the supernatant resulting from the incubation of erythrocytes in 3 mM CaCl₂ phosphate-buffered saline (PBSCa; assay no. 2). Assay no. 3 tested the nematocyst crude venom (CV) medium. Assay no. 4 tested the CV itself. Assay no. 5 tested the effect of CV in a calcium-free medium PBS.)

assay	1	2	3	4	5
	ddH2O	PBSCa	PBSCa+CV medium	PBSCa+CV	PBS+CV
haemolysis	(+)	(−)	(−)	(+)	(−)