Skip to main content
. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Transgenic Res. 2008 Dec 18;18(3):483–489. doi: 10.1007/s11248-008-9237-9

Fig 2.

Fig 2

Characterizing transcripts from the FntbΔ allele. (a) Schematic illustrating RT-PCR products obtained from Fntblox and FntbΔ alleles with an exon1/exon5 primer set and an exon1/exon8 primer set. (b) Ethidium bromide–stained 1.5% agarose gel showing the RT-PCR products generated from RNA prepared from wild-type (WT) MEFs, β-galactosidase adenovirus–treated Fntblox/Δ MEFs, and Cre adenovirus–treated Fntblox/Δ MEFs. With the exon1/exon5 primer set, the fainter amplicon between the 504-bp wild-type Fntb RT-PCR product and the 339-bp Δexon 3–4 RT-PCR product is the 412-bp Δexon 3 RT-PCR product. The numbers on the left indicate sizes of the RT-PCR products. (c) Ethidium bromide–stained 1.5% agarose gel showing the RT-PCR products (with the exon1/exon8 primer set) with RNA from wild-type (WT) MEFs and Fntblox/Δ MEFs that had been treated three times over 7 days with β-galactosidase adenovirus and Cre adenovirus. The RT-PCR reaction yields a 796-bp wild-type band and a 631-bp “Δexon 3–4” product. The numbers on the left indicate sizes of the RT-PCR products. (d) DNA sequencing chromatogram showing the sequence of the Δexon 3–4 RT-PCR product. The DNA fragment that was sequenced and purified from the agarose gel with the QIAquick Gel Extraction Kit (Qiagen). (e) Ethidium bromide–stained agarose gel showing the RT-PCR products generated from the exon1/exon5 primer set and RNA from Fntblox/Δ MEFs, the tissues of an Fntblox/Δ mouse, and tissues of an Fntblox/lox mouse. The predominant products were the 504-bp wild-type Fntb RT-PCR product and the 339-bp Δexon 3–4 RT-PCR product. The numbers on the left indicate sizes of the RT-PCR products. (f) Schematic illustrating RT-PCR products obtained from Fntblox and FntbΔ alleles with an exon1/exon4 primer set. (g) Ethidium bromide–stained agarose gel showing the RT-PCR products generated from the exon1/exon4 primer set and RNA prepared from WT MEFs, β-galactosidase adenovirus–treated Fntblox/Δ MEFs, Cre adenovirus–treated Fntblox/Δ MEFs, and tissues of an Fntblox/Δ mouse. A strong 353-bp WT RT-PCR product and a fainter 280-bp Δexon 3 RT-PCR product could be detected in tissues of an Fntblox/Δ mouse and in Fntblox/Δ MEFs. After treating the cells with Cre adenovirus, the Δexon 3 RT-PCR product was more intense. The numbers on the left indicate sizes of the RT-PCR products.