Fig. 3. The role of Gadd45gip1 in NAC-1-mediated paclitaxel resistance.
A. The level of Gadd45gip1 mRNA as determined by quantitative real-time RT-PCR was decreased in paclitaxel-resistant OVCAR3, A2780 and SKOV3 cells as compared to the parental naïve counterparts. B. The efficiency of Gadd45gip1 shRNA in reducing its mRNA level is demonstrated by quantitative real-time RT-PCR in paclitaxel-resistant SKOV3 cells. The level of Gadd45gip1 expression was normalized to that of GAPDH.
C. Reducing Gadd45gip1 by shRNA in cells increases paclitaxel resistance as compared to control shRNA treatment.