Figure 3.

Flow cytometric analysis of antibody binding to transiently transfected HeLa-MAGI cells. Constructs encoding CCR5 DNA (thick solid line) or, as a control for background staining, vector alone (shaded histogram), were transfected into the cells 2 days before staining. (A–D) Cells transfected with mouse CCR5 or vector DNA. (E–G) Cells transfected with a human/mouse CCR5 chimera (HMHH) or vector DNA. Cells were incubated with purified IgG from L1-CCR5-immunized mice (A and E), purified IgG from BPV-1 VLP-immunized mice (B and F), or purified IgG from KLH-coupled CCR5 peptide-immunized mice (D). As a control, cells also were stained with a fluorescein-labeled mAb against the second EC loop of human CCR5 (C and F).