Skip to main content
. Author manuscript; available in PMC: 2009 May 8.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):3870–3876. doi: 10.4049/jimmunol.181.6.3870

FIGURE 4.

FIGURE 4

T cells phenotype after TGF-β treatment of cells from IL-12Rβ2−/− mice and WT mice. Spleen cells from IL-12Rβ2−/− and WT mice were stimulated with anti-CD3 and anti-CD28 in the presence or absence of 5 ng/ml TGF-β for 48 h and rested in fresh medium supplemented with 50 U/ml IL-2 for 96 h. Cells were harvested and stained, and the percentages of T cell subpopulations were determined by flow cytometry. A, Number of cells per 10,000 CD4+ cells after staining. B, The fold increase was assessed by using the numbers in TGF-β-treated groups divided by the numbers in untreated groups. Columns refer to mean values and bars to SD (n = 5 each group). **, p < 0.01; *, p < 0.05. One representative experiment of three is shown.