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Sensitivity, linearity and reproducibility of glucose assay using glucose oxidase. Standards of D-glucose ranging from 0.00095 to ∼0.125 mM were linearly related to absorbance of resorufin at 560 nanometers in the presence of reagent (n = 6 replicates). The dotted line corresponds to the fit of the same glucose standards in the absence of reagent (n = 2 replicates). Inset shows regions of non-linearity of assay, starting from arrow.
