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. 2009 May 22;5(5):e1000491. doi: 10.1371/journal.pgen.1000491

Figure 3. Prdm16 and Mef2c genes are frequently insertionally mutated in AKXD Lmo2 tumors.

Figure 3

A) The Prdm16 gene was targeted in tumors 7105 and 7107. Coding exons are in yellow. Tumor 7107 had a clonal insertion as shown by the Southern blot. G shows germline bands and a red asterisk shows the rearrangement. The graph shows quantitative RT-PCR analysis for Prdm16. Real time PCR was normalized to expression in normal thymus. Tumor 7107 had a viral fusion transcript spliced from the 5′LTR to exon 2. The transcript from tumor 7105 was not recovered from the RNA since the insertion was subclonal. B) The Mef2c gene was insertionally activated in three tumors, 3095, 7105, and 98031. Southern blot using BglII-digested genomic DNA using the probe (thick line) shows all insertions were clonal and early in tumorigenesis. Quantitative RT-PCR shows the up-regulation of Mef2c.