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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Neurochem. 2009 May;109(Suppl 1):133–138. doi: 10.1111/j.1471-4159.2009.05897.x

Fig. 2.

Fig. 2

Mitochondrial PUMA upregulation after tGCI. (A) Western blot analysis shows that mitochondrial PUMA increased 4 and 24 h after tGCI, followed by cytosolic upregulation of cleaved caspase-9 and cytochrome c release. β-actin and cytochrome oxidase subunit IV (COX IV) analyses are shown as internal controls. c, control. (B) Coimmunoprecipitation analyses show that PUMA immunoreactivity precipitated by Bcl-XL or Bax increased after tGCI. Bcl-XL precipitated by Bcl-XL, and Bax precipitated by Bax were used to show equal precipitation. IP, immunoprecipitation; IB, immunoblotting. (Data modified from Niizuma et al. 2009.)