Figure 3.
Neurons in NbActiv4 increase development of NR1 and GABAA synaptic puncta faster than in Neurobasal/B27. Immunoreactive green NR1 and red GABA puncta in Neurobasal/B27 ((A), (C) and (E)) or NbActiv4 ((B), (D) and (F)) at day 7 ((A) and (B)), day 14 ((C) and (D)) or day 21 ((E) and (F)). Scale bars are 20 μm. Analysis of NR1 puncta (G) and GABA puncta (H) by density separation into four classes from region outlined in (F) (red—brightest, green, yellow, then blue—dimmest). (I) NR1 puncta densities increase linearly with development in Neurobasal/B27 (black open symbols, n = 16) or NbActiv4 (red closed symbols, n = 16). (J) Similar linear increases for GABA puncta with time in culture.