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Shank 2 expression in neuronal differentiation via ERK 1/2 activation. SNUOT-Rb1 cells were treated with 10 µM retinoic acid or 50 µM PD98059, a specific inhibitor of MEK-1. Shank 2, neurofilament, ERK 1/2 and phosphor-ERK 1/2 were detected by Western blotting analysis. Data are representative of at least three independent experiments. β-actin was served as a loading control.
