Figure 5.

Colocalization of Shank 2 with neurofilament at the contacts of outgrowing neuritis. SNUOT-Rb1 cells were treated with 10µM retinoic acid. Neuronal differentiation with retinoic acid was addressed by the morphological changes of neurite extensions. The immunocytochemistry for Shank 2 (red) and neurofilament (green) was performed, and nuclei were labeled (blue) with DAPI. Arrows indicate the colocalization of Shank 2 and neurofilament at the dendritic region of cells. Each figure is representative ones from six independent experiments. Scale bar, 10 µm.