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. 2009 Mar 31;41(3):189–200. doi: 10.3858/emm.2009.41.3.022

Figure 4.

Figure 4

Identification of interaction domains. (A) Schematic of His-tagged fragments of LCP1. (B) His-tagged fragment of LCP1 co-immunoprecipitates with FLAG-tagged PNUTS. FLAG i.p., anti-FLAG immunoprecipitate blots were probed with anti-FLAG antibody and then stripped and re-probed with anti-his antibody. LCP1 (full length) and LCP1 (415-621) were found to bind to full length PNUTS. Some of the cell lysates were immunoblotted with anti-his antibody (bottom) prior to the Flag i.p.. (C) Schematic of Flag-tagged fragments of PNUTS. (D) Flag-tagged fragment of PNUTS co-immunoprecipitates with His-tagged LCP1 (full length). FLAG i.p., anti-FLAG immunoprecipitate blots were probed with anti-His antibody and then stripped. Some of the cell lysates were immunoblotted with anti-His antibody (bottom) prior to the FLAG i.p.. PNUTS (full length) and PNUTS (1-260) were found to bind to LCP1 (full length). After the FLAG i.p., some of the cell lysates were immunoblotted with anti-FLAG antibody. (E) Schematic of putative interaction region between PNUTS and LCP1 protein.