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. Author manuscript; available in PMC: 2009 May 8.
Published in final edited form as: Circulation. 2008 Jun 2;117(24):3099–3108. doi: 10.1161/CIRCULATIONAHA.108.767673

Figure 3.

Figure 3

Fstl1 is secreted from cells. A, HEK293 cells were transfected with a plasmid expressing V5 epitope-tagged Fstl1 protein. Western blot analysis using anti-V5 antibody indicates that Fstl1 is present in the cell pellet and the culture media. B, NRVMs were transfected with an adenovirus expressing Fstl1 or β-galactosidase. Western blot analysis using anti-Fstl1 antibody revealed Fstl1 protein expression in the cell pellet and the culture media, and the intensity of these bands increased in cells that were transduced with Ad-Fstl1.