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. Author manuscript; available in PMC: 2009 May 8.
Published in final edited form as: Circulation. 2008 Jun 2;117(24):3099–3108. doi: 10.1161/CIRCULATIONAHA.108.767673

Figure 5.

Figure 5

Fstl1 inhibits hypoxia/reoxygenationinduced apoptosis. A, Serum-deprived NRVMs underwent 12 hours of hypoxia followed by 24 hours of reoxygenation. Representative photographs of cultures stained with TUNEL (green; top panels) to identify apoptotic nuclei and DAPI (blue; bottom panels) to identify total nuclei are shown. Cultures were transfected with Ad-Fstl1 or Ad-βgal before hypoxia/reoxygenation. Scale bar=100 μm. B, Quantification of TUNEL-positive cell number. *P<0.01 compared with Ad-βgal-transfected NRVMs after hypoxia/reoxygenation (H/R). C, Nucleosome fragmentation enzyme-linked immunosorbent assay shows that transduction with Ad-Fstl1 reduced apoptosis after hypoxia/reoxygenation. *P<0.01 compared with Ad-βgal-transfected NRVMs after hypoxia/reoxygenation.