Figure 2.

a, Movement of wild-type embryo. Sequential images showing movement of a 3dpf wild-type embryo in response to tactile stimulation. Each panel represents images taken at 0, 10, 20, 30, 40, or 50 ms after the initiation of movements. A montage image of the same embryo at 0 and 200 ms in a wider view is shown at the bottom. b, Movement of δ2YFP-injected sop^−/− embryo displayed in the same manner as a. c, Sop^−/− embryo exhibits no voluntary movement at 0, 50, or 200 ms. d, Expression of δ1/2YFP in injected sop^−/− embryo. YFP fluorescence in a sop^−/− embryo expressing δ1YFP (top) or δ2YFP (bottom) at 3 dpf. Embryos were injected at the 1 cell stage. Fluorescence in the eye (arrowhead) and yolk sack (arrow) is endogenous. YFP signal resulting from the expression of δ1/2YFP is detected in muscle cells in the trunk. Expression occurs in a stochastic manner, and the fluorescence is mosaic.