Figure 6.

a, A 1-year-old δ2YFP/sop^−/− fish. The fish is anatomically indistinguishable from wild-type fish. Scale bar, 1 cm. b, The δ2YFP/sop^−/− fish was genotyped with BccI and direct sequencing. The chromatogram of sequencing results is shown, which is complementary to the coding strand. This fish shows G at the critical site (marked with an asterisk; top), whereas a heterozygous fish shows a mixture of A and G (middle), and a wild-type fish shows A (bottom). c, Behaviors of a δ2YFP/sop^−/− fish. In the top, it is making a C-bend. In the bottom, it is eating a flake of food on the water surface. d, YFP fluorescence detected from a transverse section of muscle in δ2YFP/sop^−/− fish. The left panel is a merged image of the YFP signal and transmitted light. The middle panel is a merged image of the α-Btx signal and transmitted light. The right panel is the merged image of all signals.