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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 22;1794(2):211–224. doi: 10.1016/j.bbapap.2008.09.025

Figure 2. Induced α-Syn expression leads to accumulation of α-Syn and its derivatives exhibiting different isoelectric points and apparent molecular weights.

Figure 2

SN1 fractions from 1.5 × 106 3D5 cells with or without TetOff induction of α-Syn were separated by 2-D gel electrophoresis and analyzed by Western blotting using Syn1 antibody. S-14d or S-28d: without induction for 14 days or 28 days. S+14d or S+28d: with induction for 14 days or 28days. In the S-14d the spot shown at pI 10 and migrated around 50 kDa is unrelated to α-Syn, since it was not recognized by other antibodies to α-Syn (n=3).