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. 2008 Aug 31;40(4):361–369. doi: 10.3858/emm.2008.40.4.361

Figure 3.

Figure 3

TUNEL staining of FKCs cultured in (A) DMEM and (B) KSFM. The red fluorescence indicates TUNEL-positive apoptotic cells, and the blue fluorescence indicates a cell nucleus. Arrows indicate TUNEL-positive cells. Scale bars indicate 25 µm. (C) Percent of apoptotic cells in FKCs cultured in DMEM (solid bars) and KSFM (open bars). P < 0.05 between any two groups. (D) mRNA expression levels of the apoptosis-related genes, Bax and Bcl-2, in cultured FKCs . M: 100 bp DNA molecular weight marker.