Figure 1.

Expression of CAR and αν integrin in cancer cell lines after Ad-GFP treatment. (A) Expression levels of CAR and αν integrin mRNA transcripts in mouse cancer cell lines. Total RNAs were isolated from each cell lines and RT-PCR was performed with mouse GAPDH as a control. PCR products were separated on 1.2% agarose gels and stained with ethidium bromide. (B) Adenoviral gene expression in CAR-positive and -negative cell lines. Mouse cancer cells were seeded in six-well plates and infected with 30 MOI of Ad-GFP to Hepa1-6 cells or 200 MOI of Ad-GFP to CT-26, NIH3T3, and B16F10 cells. After 1 day, GFP expression in each cells was determined at 40× magnification taken using fluorescent microscope.