Figure 2.
Effect of liposome dose and adenovirus concentration on efficacy of liposome-complexed adenoviral gene expression. (A) Effect of liposome dose on adenoviral gene expression in CT-26 cells. Varying amounts of PEGPE liposome were incubated with concentration of Ad-GFP (1 × 102 pfu/µl) for 30 min and then added to CT-26 cells. After 24 h, the percentage of GFP expressing cells was determined by FACS analysis. The data represents mean ± S.D. of triplicate experiments. (B) Effect of adenovirus concentration. Varying concentrations of Ad-GFP were incubated with PEGPE liposome at fixed liposome dose (2.5 µl). The next experimental procedures were the same as (A).