Figure 1.

Scavenger activity of NDGA, LOXs inhibitor, in lymphoid cells. Raw 264.7 cells, THP-1 cells, Jurkat cells, BEAS 2B cells were treated with or without 5 µM NDGA for different times, as indicated. The cells were treated with 20 µM DCFH and incubated for 30 min at 37℃. The levels of ROS were determined via flow cytometry (A, RAW 264.7 cells; B, THP-1 cells; C, Jurkat cells; D, BEAS 2B cells). Raw 264.7 cells were treated with different concentrations of REV5901 for 60 min, as indicated (E), or 50 mM REV5901 for different times, as indicated (F). The cells were treated with 20 µM DCFH and incubated for 30 min at 37℃. The levels of ROS were determined via flow cytometry. Data are representative of three individual experiments. Decreases in mean fluorescence intensity were represented in the right panels. The bar represents the mean ± SE from three individual experiments. ^*P < 0.05 vs control (0 min).