Figure 5.

NDGA reduces inflammatory cytokines and the number of cytokine-producing cells. (A) Mouse BAL fluid samples from mice treated as described in the Figure 3 legend were collected 24 h after the last challenge. Enzyme immunoassays for IL-4, IL-5, IL-13, and TNF-α were conducted as described in the Materials and Methods section. Bars represent mean ± SE from 6 independent experiments. ^*P < 0.05 vs OVA + VEH mice. (B) Mouse splenocytes from mice treated as described in the Figure 3 legend were harvested 24 h after the last challenge. For the detection of numbers of IL-4, IL-5, IL-13, and TNF-α-producing cells, ELISPOT assays were conducted as described in the Materials and Methods section. Spots were counted using Elispot leader 4.0. As a negative control, unstimulated cells were used. No spots were detected in the control wells. Bars represent mean ± SE from 6 independent experiments. ^*P < 0.05 vs OVA + VEH mice.