Figure 3.

ACAT inhibition regulates the expression of genes involved in cholesterol catabolism and mobilization in cultured macrophages. Cells were incubated for 48 h with acLDL and the indicated concentration of OAA. (A) The levels of mRNA were measured by qRT-PCR and normalized by β-actin. ^*P < 0.05 versus acLDL-loaded cells. (B) Each protein expression level was analyzed by Western blot. The intensity of the detected bands was compared using a Calibrated Densitometer GS800 (Bio-Rad) with Quantity One software (version 4.4.0) and demonstrated as a average of three independent experiment. The intensity of the β-actin band was used as an internal control. ^*P < 0.05, ^**P < 0.01 versus acLDL-loaded cells.