Figure 4.

ΔNDg3 regulates migration of epithelial cells. (A) HaCaT cells were transfected with control vector (pcDNA3.1) or κNDg3 expressing construct (pcDNA3.1-ΔNDg3). Scratch wounds were made using a pipette tip, then cells were incubated for 18 h. A cell-free area was photographed and measured by image analyzer. Data are represented as percent control and SEM, measured from three independent experiments (^*P < 0.05). (B) Control and ΔNDg3 transfectants were grown to confluence. Monolayers were separated from culture dishes via incubation with dispase. Dissociation assay results were quantified by counting the number of total particles. Data represents the average and SEM from three independent experiments (^*P < 0.05).