Figure 1.

T-cell specific disruption of BRCA2 using Cre-loxP recombination system. (A) Schematic representation of wild type and mutated BRCA2 alleles. Two loxP sites as triangle flanks exon 11 of BRCA2 (BRCA2^F11). Recombination by Cre recombinase can induce excision of exon 11 (BRCA2^Del11). Small arrows indicate PCR primer sites for mouse genotyping. (B) General genotyping pattern using PCR screening. Using primer 10F and 10R in Figure 1A, wild-type allele is represented as 298 bp band, but band of BRCA2^F11 is shifted to 376bp, since additional loxP sequences add up.