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Characterization of TrkA-inducible stable cell lines. (A) U2OS-TrkA (#8, #60, #67) cells were cultured for 12 h in the absence or presence of tetracycline. (B) U2OS-TrkA-60 cells were uninduced or induced with tetracycline for 12 h and untreated or treated with doxorubicin for 4 h. Proteins from the cells were extracted with SDS sample buffer and analyzed by Western blot with the indicated antibodies (A, B).
