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Identification of the HSP27 Ser135Phe mutation. (A) Sequencing chromatograms showing the C404T (Ser135Phe) mutation in HSP27 exon 2. Exons were amplified by standard PCR and sequenced using an ABI 3100 automatic sequencer. (B) Conservation of an amino acid at the Ser135Phe mutation site in different species. Multiple alignments of HSP27 proteins were performed using the ClustalX 1.83 program. The mutation is located on the Hsp20-α-crystallin domain.
