Figure 3.

Cancer-specific activation of the reporter gene by both PRC1 and RRM2 promoters. pmGL3-PRC1, -RRM2, -BIRC5, TERT or empty pmGL3 (pGL3-basic) vector was transiently transfected into normal (A: MCF10A) or cancer (B: MCF7; C: MDA-MB-231 or D: T47D) breast cell lines. pRL-TK was also co-transfected to normalize transfection efficiency except MCF10A. For MCF10A cells, only reporter plasmids were transfected and the average of three separate experiments with triplicates was shown. Forty-eight hours after transfection, cells were harvested and their luciferase activity was measured. The transfection experiments were carried out more than twice in triplicate and one representative result was shown. The CMV promoter was included in MCF10A cell line to confirm its strong activity in the normal cell line. In T47D cell line, TERT was omitted.