Figure 2.

Purified CBZ-lysine AP was incubated for up to 4 weeks in the absence (○) or the presence of 4 (▴) or 20 (▾) equivalents of acetaldehyde (12). At intervals, aliquots of each incubation mixture were analyzed for the development of AGE-associated absorbance changes (Top) and for immunoreactivity in independent competitive ELISAs employing two different AGE-specific antibodies: a polyclonal antibody raised to AGE-modified RNase A (Middle), and a monoclonal antibody raise to AGE-modified KLH (Bottom). The values displayed show the mean ± SD of triplicate measurements.