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Down-regulation of LARS1 expression in A549 cells by treating siRNA against LARS1 (siLARS). (A) A549 cells were transfected with 50 nM of siLARS or siCon and incubated for 14 hours. Then LARS1 expression was compared between siLARS and siCon treated cells by qRT-PCR as described in Materials and Methods. As an internal control, GAPDH mRNA was used. (B) LARS1 protein expression was compared between the same cells by immunoblot analysis. β-actin was probed for equal protein loading.
