Skip to main content
. 2008 Feb 20;40(1):98–108. doi: 10.3858/emm.2008.40.1.98

Figure 2.

Figure 2

Differences of BrdU incorporation, CyclinD expression and telomerase activity in hESCs according to culture period. (A) BrdU incorporation assay of early, middle and late group in hESCs. Cells were treated with 10 µM of BrdU for 24 h. After incubation, samples were trypsinized to dissociate into single cells and labeled with BrdU antibody. Expressions of Cyclins were analyzed using quantitative real-time PCR. mRNA expression levels were normalized to GAPDH expression. Relative gene expression was determined by normalization to that of early passage group. (B) Telomerase activities in each group of hESCs. Telomerase activities of early, middle and late passaged hESCs were analyzed using TRAP assay. Heat-inactivated controls were heat-inactivated at 85℃ and loaded beside each sample. E, early passage; M, middle passage; L, late passage; D, differentiated hESCs; lane 1, 3, 5, 7, untreated hESCs; lane 2, 4, 6, 8, heat-inactivated hESCs; PC, positive control provided by kit; N, negative control.