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RT-PCR for the expressions of undifferentiated state markers from each group of hESCs and EBs. (A) Expression levels of Oct4 and Nanog in human embryonic stem cells (ES), embryoid bodies (EB) and reattached embryoid bodies (EB-AD). (B) Expression levels of Oct4 and Nanog in simple and cystic EBs from each passage group. E, early passage; M, middle passage; L, late passage; -RT, PCR without addition of reverse transcriptase.
