Skip to main content
. 2008 Feb 20;40(1):98–108. doi: 10.3858/emm.2008.40.1.98

Figure 7.

Figure 7

Immunostaining and RT-PCR of three germ layer markers in reattached EBs from each group of hESCs. (A) Expressions of Nestin, Brachyury, HNF3β in reattached EBs were observed by confocal microscope. (B) Expressions of Nestin, Brachyury, HNF3β in reattached EBs were analyzed by RT-PCR. Expression level of three germ layer markers were detected in reattached EBs from each group of hESCs. E, early passage; M, middle passage; L, late passage; -RT, PCR without addition of reverse transcriptase.