Figure 3.

Serum PrV-specific IgG levels and associated isotype distributions in animals immunized by rAd-gB systemic and mucosal delivery. Groups of C57BL/6 (H-2b) mice were immunized with rAd-gB (10⁶ pfu/mouse) by either the i.m. or i.n. route three times at 7 day intervals. On the seventh day after each immunization, the PrV-specific IgG levels in the sera were evaluated by ELISA. The levels of IgG2a and IgG1 were compared 7 days after the final immunization. The circles on the graph represent the individual IgG levels and the height of the bar shows the average of each group (n = 7). P-values in the graphs were calculated using the Student's t-test.